Hzymes Recombinant Trypsin -lyophilized powder/ solution / digestion CAS NO.9002-07-7

The Chinese Pharmacopoeia recommends the use of biomaterials produced by recombinant technology to replace animal-derived biomaterials (2020 edition, PartII,page 25).Recombinant Trypsin is widely used in production and research process.

No Animal origin: Recombinant trypsin is free from exogenous virus contamination.Trypsin extracted from porcine and bovine pancreas has the riskof viral infection. It is necessary to detect the content of viruses (such as porcine parvovirus, etc.) in nature trypsin.

Process stability: Recombinant trypsin process is stable,and little between batches to batch difference.

High activity: According to the test, the specific activity of recombinant trypsin is about 5 times higher than animal-extracted trypsin

Digestion termination: Recombinant trypsin can be directly diluted with PBS to terminate digestion in the laboratory. Soybean trypsin inhibitor can be added to terminate digestion during large-scale production to avoid fatal change caused by complex components in serum.

High yield: Recombinant trypsin is expressed through recombinant E. coli or Pichia pastoris cells, which have access to mass-production. 

Trypsin specifically cleaves C-terminal peptide bonds of lysine and arginine, which can degrade intercellular binding proteins. The amino acid

sequence of the recombinant trypsin produced by Hzymes Biotech is the same as that of porcine pancreas-derived trypsin, and is produced by

recombinant Escherichia coli expression. It can replace traditional extraction of trypsin for cell digestion in vaccines, stem cells, immune cell

therapy, drug screening, antibodies and other fields. Aprotinin, soybean trypsin inhibitor, etc. can distinctly inhibit trypsin activity.

Recombinant trypsin has the same properties as naturally extracted trypsin. This product is manufactured in a GMP grade workshop. No animal origin raw materials are used in the fermentation, purification and final ingredients.

At the same time, the production and quality control processes are validated, resulting in a high level of consistency between batches.