Hot Start PCR Master Mix (Chemically modified,2×mix)

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Taq DNA polymerase is a heat-resistant DNA polymerase derived from Thermus aquaticus YT-1. The enzyme has a polymerase activity of

5'→ 3'and a specificity of 5'→ 3 for the double-strand 'Exonuclease activity. The enzyme uses a chemical modification group to block the

enzyme activity at low temperature and room temperature. After thermal activation at 95°C, the chemically modified group falls off to

release the enzyme activity. Before the amplification system is formulated to PCR amplification, non-specific amplification due to

primer mismatch will not occur, which minimizes the formation of non-specific amplification and primer dimers, and improves

the sensitivity and specificity of amplification. This product uses a specially optimized reaction buffer and hot-start Taq DNA polymerase

(chemically modified) to make a premixed reagent, which maximizes the stability of the enzyme during storage. Only the primer

template and water are added before configuring the system. , Greatly simplifies the preparation steps. This product has strong versatility and is

suitable for end-point PCR, dye and probe qPCR.